FastQCFastQC Report
Sun 14 Apr 2019
SRR6576422_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576422_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206435
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5030.24366023203429651No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3590.1739046188873011No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3400.1647007532637392No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3150.1525904037590525No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2660.12885411872986655No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2660.12885411872986655No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2410.11674376922517983No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2300.11141521544311768No Hit
GGAGAAGGATACTCATTGATTCTCCTGGGCCACTGGGGTGATGATGGGGT2170.10511783370068059No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2100.10172693583936833No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATAC405.6712405E-446.9909133
TAGGTTT450.001006958341.8203694
CATGGGT3400.038.69844
GTATCAA8000.038.2542571
GTATACG250.00169934337.60639666-67
AGTATAT651.4110803E-436.1468542
GCGTTAT550.002688735334.241571
GCAACAC550.00271444234.1752133
ATGGGTA1606.002665E-1132.3062555
GTACATG14250.031.3881071
GAGTATA753.2330074E-431.3881071
GTATAAG600.004117781831.3881071
TCTAGTC300.00416185731.32727848-49
CGTTATT600.00415708131.3272782
AACGCAG9900.031.3272786
TACATGG14300.031.2177412
ACATGGG14600.030.5762833
GTGTTTA804.7189268E-429.426351
ATGGGTG1452.6428097E-829.1667735
CCCCTAT650.00609042128.9736351