FastQCFastQC Report
Sun 14 Apr 2019
SRR6576423_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576423_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169369
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3990.2355803009995926No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2420.14288329033057998No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2300.13579816849600576No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2110.12458005892459659No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1820.1074576811577089No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1760.10391512024042181No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATA250.005217700756.4290662
GTATATA451.6031627E-552.2491381
GTATCAA3650.041.226721
ATAAGGA608.796146E-539.1637153
CATTATA651.4041422E-436.172482
GTCCTAG550.00270365834.1994361
ACATGGG9450.032.822923
GTACATG9850.031.5086171
ATGGGGG1950.031.3309735
AACGCAG4650.031.3309736
TACATGG10150.030.577332
ATCAACG4700.029.997743
GGTACCT1602.1082087E-929.3727878
CAACGCA4900.028.7733385
TCAACGC5000.028.1978744
CATGGGG4350.028.0898364
GGGTACC1704.0272425E-927.6449747
TAGTTAG856.794941E-427.64497494
ATGGGTA1904.4201443E-1027.2084755
GGCTAAT700.00879301326.8709831
Produced by FastQC (version 0.11.2)