FastQCFastQC Report
Sun 14 Apr 2019
SRR6576423_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576423_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169369
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3850.2273143255259227No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2610.15410139990198915No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2590.15292054626289345No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2090.1233992052855009No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2070.12221835164640518No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1890.11159066889454387No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1830.10804810797725675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA3300.058.646331
ACATGGG9350.038.2209783
CAACGCA4950.037.9974635
ATCAACG4950.037.9974633
GTACATG9600.037.8608551
GTATTGA500.001662320237.7625161
GTCTTAG500.001662320237.7625161
TATAAAC500.00169405637.617492
ACCGTCG757.4125073E-637.5952768
TCAACGC5150.037.434884
TACATGG9900.037.0475272
AACGCAG5200.036.1706626
ATACCGT651.4045573E-436.1706626
CCGTCGT801.1538266E-535.2455759
ATGGGAG1900.034.6476865
AATAGTA550.002704325134.1977162
ACGCAGA5800.032.409727
ATGGGTA1605.820766E-1132.327535
CATGGGT2500.031.9748674
CATGGGA4050.031.3479084
Produced by FastQC (version 0.11.2)