FastQCFastQC Report
Sun 14 Apr 2019
SRR6576424_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576424_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152910
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3860.25243607350729186No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2240.14649140017003465No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1960.12817997514878032No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1950.12752599568373554No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1950.12752599568373554No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1780.11640834477797397No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1720.11248446798770519No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1700.1111765090576156No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1680.10986855012752599No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1610.1052906938722124No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG1530.10005885815185403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGT200.002161680570.4811294
GCTAGAC250.005232931656.3848952
TAGTACA352.933324E-453.6998989
CATGGGG3550.043.678444
TCTAATA450.001012530441.766592
GTACATG8800.039.5121421
ACATGGG8700.039.426223
TACATGG8850.039.288912
TAGCGCG500.001699651637.5899284
GTATCAA3000.037.5899281
ATGGGGC1204.1673047E-935.240565
GTGTTAG550.00271324334.172661
TCTAGCC550.00271324334.172663
TAACACA550.00271324334.172664
AGCGCGC550.00271324334.172665
ACATACT550.00271324334.172665
TGTAGCG702.1796161E-433.5624352
CTGGTAC851.7480985E-533.1675833
GCGCGTG600.004155256331.3249428
CGCGCGT600.004155256331.3249427