FastQCFastQC Report
Sun 14 Apr 2019
SRR6576424_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576424_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152910
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3700.2419724020665751No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2380.1556471126806618No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2350.15368517428552744No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1980.1294879340788699No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1980.1294879340788699No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1900.12425609835851155No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1870.12229415996337714No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1800.11771630370806356No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1780.11640834477797397No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1710.11183048852266039No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1650.1079066117323916No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT1560.10202079654698844No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCAG250.005231275756.3894045
GTATCAA3850.041.580281
GTACATG7750.036.451781
GTATAAT651.3931238E-436.218121
TACATGG8200.034.9568442
TCAACGC4550.034.081514
ATCAACG4600.033.7110563
ACATGGG8450.033.3665123
CAACGCA4700.032.99385
ATATCTT753.2659763E-431.3274485
TATAGGC753.2659763E-431.3274483
CATGGGG4350.031.3274464
AACGCAG5000.031.0141726
ATTAGTG804.7669848E-429.3694845
TAGGGAG650.006143358528.9176435
TGTACTG650.006143358528.9176435
ACAACCG700.00882059626.8520955
GTCACGA350.00883415926.85209544-45
ACGCAGA5800.026.7363577
TATCAAC5850.026.5078412
Produced by FastQC (version 0.11.2)