FastQCFastQC Report
Sun 14 Apr 2019
SRR6576425_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576425_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533833
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6620.12400881923747689No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5660.10602566720303915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12450.041.9265941
ATCAACG14700.035.48933
TCAACGC14950.034.5814554
CAACGCA15500.033.6575975
GTATAAC1151.12991984E-732.713581
AACGCAG16100.032.6951946
TATCAAC16900.030.5941542
GTACTAC1303.3100332E-728.9389381
ACGCAGA18400.028.6082977
GTACATG24200.026.8163491
TACATGG23950.026.6910632
CGCAGAG20300.025.9306748
ACATGGG24400.025.8111153
GTCTAAA1501.1550474E-625.0804121
CCTATTG950.001303776624.73649694
GCAGAGT21800.023.9308579
GGTATCA6600.023.5128881
CATGGGG13900.023.330614
GTACTAG1652.6467642E-622.8003751
GATATAT1252.4121057E-422.572371
Produced by FastQC (version 0.11.2)