FastQCFastQC Report
Sun 14 Apr 2019
SRR6576426_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576426_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267578
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3930.14687306131296293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGCG352.9339496E-453.7123649
GTACATG16150.037.263371
TACATGG16050.036.6167372
ACATGGG16000.036.1299553
CATGGGG5250.031.3322124
CATGGGT4000.030.5489064
ATGGGAG3150.029.84025
GTATCAA7600.028.4569841
GGGTACC1853.255991E-1027.9449467
GGTACCT1904.4383341E-1027.209558
GTTCTAG1406.318969E-726.8662241
CATGGGA9100.026.8561824
CGTCTAC350.0088334226.85618216-17
ATGGGGG2800.025.177675
TAATACT950.001302243924.7359544
CTATACT950.001302243924.7359544
AGCGCGC950.001302243924.7359545
ATGGGTA2701.8189894E-1224.36955
ATCAACG9100.023.7573913
CTATGGT1000.001755535523.4991594