FastQCFastQC Report
Sun 14 Apr 2019
SRR6576426_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576426_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267578
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3560.1330453176270097No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.11548034591782584No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGGG250.005235451756.3896837
GTACTAG704.556845E-640.3688771
TACATGG15500.035.780792
GTACATG15700.035.6977421
GTATCAA7450.034.7696151
GATATAA550.002685995834.252381
GTGTAAT702.1542796E-433.640731
ACATGGG16250.032.972443
CTAATAG600.00415524931.3334583
AACGCAG9250.028.9622766
ATTAGTG650.00614572728.923193
GTATTAC650.00614572728.923193
CATGGGT3000.028.2001134
ATGGGGG2500.028.2001115
CAACGCA9600.027.4167755
ATCAACG9300.027.2904323
CCTATAC700.008823954526.857253
GTCGCTA350.00883975926.85223224-25
GAGCCGA1057.4098934E-526.8522329
GCAGCGT700.00883200326.8522328
Produced by FastQC (version 0.11.2)