FastQCFastQC Report
Sun 14 Apr 2019
SRR6576427_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576427_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238620
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3510.14709580085491575No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3310.13871427374067555No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2750.11524599782080296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8050.040.9466251
AACGCAG9250.034.0359576
ATCAACG9800.033.5642433
CAACGCA10200.032.2479975
TACATGG13850.032.2313652
TCAACGC10250.032.090694
ACATGGG13750.031.4405423
TAGGAGT1052.059749E-631.3266284
GTACATG14000.031.2802941
TATCAAC11100.030.056632
ATCTAGT804.7173223E-429.4303871
GTATAAG650.006088220528.977611
CGCAGAG10900.028.9018968
ACGCAGA10900.028.8837247
CATACTG856.808671E-427.6411445
GTTCCGG700.00879540826.8739294
TAAACCT1057.408159E-526.8513954
CATGGGA7500.026.3143674
GCAGAGT12150.025.5414589
AGTAATA1151.3747421E-424.516492
Produced by FastQC (version 0.11.2)