FastQCFastQC Report
Sun 14 Apr 2019
SRR6576428_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576428_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231089
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4330.18737369584878552No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2990.1293873788886533No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2780.12029997100684153No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.10861616087308353No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2390.10342335636919109No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2370.10255788895187569No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2320.10039422040858717No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAC405.653428E-447.023281
GTATCAA6250.045.894721
TACATGG18050.037.5144162
GTACATG18250.037.3609621
ACATGGG18500.036.8321573
ATATAGG550.002714591334.1765443
TATAGGT702.1815996E-433.566254
AACGCAG8550.032.9773676
CAACGCA8650.032.5961275
CATGGGT3650.032.1868174
TCAACGC8850.031.859494
ATGGGAG4600.031.6690275
ATCAACG8950.031.5035213
CATGGGG7200.031.3284974
ATGGGAT2200.029.9044785
CATGGGA10250.029.800284
ATGGGTA2550.029.485655
GTGTAAG804.7553878E-429.3895511
CGGCTCG804.7735128E-429.370478
GGGTACC1853.255991E-1027.9416357
Produced by FastQC (version 0.11.2)