FastQCFastQC Report
Sun 14 Apr 2019
SRR6576428_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576428_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231089
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5540.23973447459636762No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3400.14712946094361914No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3160.13674385193583424No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.12549277551073396No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2830.12246363955013004No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2550.11034709570771435No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2510.10861616087308353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6950.044.1624341
GAGTCGG459.892845E-441.9731831
TACATGG17500.040.290172
GTACATG17800.039.7919921
ACATGGG17900.039.3898283
ATGGGAG3600.037.85715
CATGGGT3750.036.3506854
CATGGGG5950.035.5501484
ATCAACG8850.034.523593
TCAACGC8850.034.523594
TATGGGT550.002711067734.1855964
CAACGCA9250.033.023535
ATGGGTA2150.032.7872245
AACGCAG9450.032.324626
CATGGGA10250.031.6425194
GTTGTAA753.179264E-431.4798851
CCGTTCA600.00415632131.3300179
GGTACCT2250.029.2413488
TATCAAC10450.029.237682
ACGCAGA10900.028.0245557
Produced by FastQC (version 0.11.2)