FastQCFastQC Report
Sun 14 Apr 2019
SRR6576429_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576429_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208384
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4620.22170608108108109No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2730.13100813882063883No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2540.12189035626535627No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2500.11997082309582309No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.10557432432432433No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2110.1012553746928747No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAC451.6074307E-552.232761
GTATCAA7100.039.7263261
GTACATG15300.038.713691
TACATGG15650.037.8478932
ACATGGG15450.037.711193
GATATGC500.001701155437.5895356
CATACCC550.002715632834.1723064
AACGCAG8300.033.966456
ATCAACG8400.033.002723
CAACGCA8550.032.9732785
ATGGGAG3450.032.6865545
TCAACGC8700.032.4047744
TATAGTC600.00414913631.3396552
GTATAGT600.00414913631.3396551
CATGGGT2700.031.3246144
CATGGGG5400.031.3246144
GTGAGTA600.004158900631.3246144
TCTATAC600.004158900631.3246143
ACGCAGA9500.029.1813537
GATTTAT650.006136710828.9289131