FastQCFastQC Report
Sun 14 Apr 2019
SRR6576429_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576429_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208384
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4210.2020308660933661No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3400.16316031941031942No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3100.14876382063882063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.1401259213759214No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2620.1257294226044226No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2570.12333000614250614No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2340.11229269041769041No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2280.10941339066339066No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2230.10701397420147421No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG2200.10557432432432433No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2130.1022151412776413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6850.041.937861
GTCCTAT853.8736107E-738.783421
ACATGGG16400.037.8231853
TACATGG16600.037.650572
CACCGTC500.001700174237.5939527
GTACATG16850.037.4517331
CATGGGG5350.034.2561724
CTATTCC1107.6557626E-834.176324
CCTATTC1107.6557626E-834.176323
TTCATAC702.180952E-433.5660253
TCAACGC8550.033.5267684
ATCAACG8600.033.331853
AACGCAG8750.032.7604456
ATACAGT1309.12587E-932.533236
CAACGCA8950.032.0283665
CATGGGT3000.031.3282934
GTATTTG804.712041E-429.4338471
GGGGCTA650.006147597528.9184255
GTCTTAG1005.2310475E-528.2564931
TATCAAC10500.028.1954652
Produced by FastQC (version 0.11.2)