FastQCFastQC Report
Sun 14 Apr 2019
SRR6576431_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576431_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232781
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4940.212216632800787No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3100.13317238090737646No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3080.13231320425636114No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2560.1099746113299625No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2520.10825625802793183No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2430.10438996309836285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAG205.658779E-447.024384-85
TGCGACG205.682814E-446.9838922-23
ATAGTCC608.8201996E-539.153248
ACTAGGC757.424589E-637.595193
GTACTAG651.4057464E-436.1725351
TACATGG16700.036.0425722
GTATCAA9050.035.3331761
GTACATG17350.034.963311
ACATGGG16750.034.7895773
GAGTAAG550.002705710234.199491
GTATACA550.002705710234.199491
CTAGCAC550.002714281434.177443
TCGTTCG753.2741594E-431.3225929
TACGCCA300.00416544531.32259252-53
CGCACTA300.00416544531.32259258-59
CATGGGA9350.030.6591764
ATGGGAC1252.3565735E-730.0696895
ATGGGAG4150.029.4356885
GTGTAAT804.7548895E-429.3901861
CTACACC804.7728835E-429.3712434
Produced by FastQC (version 0.11.2)