FastQCFastQC Report
Sun 14 Apr 2019
SRR6576432_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576432_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275827
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8460.3067139910161079No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5090.18453595913380488No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4590.16640865470022875No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4260.15444463377406853No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4160.1508191728873533No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3970.14393079720259438No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3700.13414205280846328No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3410.12362821623698912No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3400.1232656701483176No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3160.11456456402020106No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3050.11057655704481432No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3020.10948891877879975No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2970.10767618833544214No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2960.10731364224677062No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2810.10187545091669778No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2790.10115035873935474No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA2780.10078781265068322No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2760.10006272047334017No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACA801.1559374E-535.247894
GTATCAA8950.032.574431
ACATGGG16050.031.6242793
AACAGCG600.004156766531.331467
GTACATG17000.030.7030981
TACATGG16700.030.6915042
CATGGGC2500.030.0782034
TAGTTCC1252.3534085E-730.07820394
CATGGGG5650.029.945114
CATGGGT3600.028.7205054
AACGCAG9850.028.627736
GGGTACC2350.027.9983277
ATGGGCA1853.255991E-1027.9442755
ATGGGTA3050.027.7360485
ATCAACG9850.027.6734713
TCAACGC10100.027.4538024
CAACGCA10200.027.1846475
GATATAG1057.377127E-526.8701521
GTATATG1257.965018E-626.332751
CTCTATA909.4724615E-426.1237582
Produced by FastQC (version 0.11.2)