FastQCFastQC Report
Sun 14 Apr 2019
SRR6576432_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576432_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275827
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8190.2969252466219768No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4960.1798228599810751No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4290.1555322720400831No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4260.15444463377406853No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3690.13377950671979175No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3650.13232932236510567No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3340.12109039361628848No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3330.12072784752761695No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3230.11710238664090172No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3190.11565220228621563No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3180.11528965619754411No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3060.11093910313348584No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2980.10803873442411366No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA2760.10006272047334017No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGA451.6112048E-552.2196049
ATAGACG451.6112048E-552.2196048
ATGGGTA2950.043.014795
GGGTACC2900.040.515217
TAGGCAT608.809248E-539.16475
CATGGGT4050.038.2943764
GTATCAA9400.036.624321
TGGGTAC3250.036.1520356
GTATAGA550.002668566534.298251
TGCGGAG550.002714112934.1801072
GTACATG18250.031.7846071
TACATGG18450.031.3317622
CATAAGT600.004156571331.331764
ACATGGG18150.030.8138833
TCAACGC11350.030.6416344
GGTACCT3550.030.4491778
ATCAACG11450.029.5531023
CATGGGG6700.029.461214
CAACGCA11650.029.4491675
AATAGAC804.772977E-429.3735267
Produced by FastQC (version 0.11.2)