FastQCFastQC Report
Sun 14 Apr 2019
SRR6576433_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576433_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247547
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8800.3554880487341798No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4670.18865104404416133No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4530.1829955523597539No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4190.1692607868404788No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3900.15754583977992057No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3540.14300314687715868No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3290.13290405458357402No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3200.12926838135788354No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3140.12684459920742325No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2910.11755343429732536No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2890.11674550691383857No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2890.11674550691383857No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2820.1139177610716349No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2780.11230190630466133No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2720.10987812415420102No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2640.10664641462025393No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2610.10543452354502378No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2610.10543452354502378No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2560.10341470508630683No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2550.10301074139456347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGT3150.038.790484
ATGGGTA1850.038.1049965
GTACATG18050.036.4660531
ATGGGAT2200.036.3152165
TCTAGAC651.4109653E-436.150893
TACATGG18450.035.937552
ACATGGG18400.035.5025373
CATGGGG6000.033.6805844
GGTACCT1900.032.1552668
CTTATAA600.004148176431.3434431
GGGCCTA600.00415639431.3307747
ATAGTAC753.269742E-431.3307743
GTATAGG953.732289E-529.6937851
TGGGTAC2250.029.2420566
GTATCAA10500.028.656861
GGGTACC2203.6379788E-1227.770467
ATGGGAG2550.027.64485
TATAAGG1205.795222E-627.4310572
CTCACGC350.00884347726.84952562-63
CATGGGA10800.026.5441284
Produced by FastQC (version 0.11.2)