FastQCFastQC Report
Sun 14 Apr 2019
SRR6576433_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576433_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247547
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8030.32438284446993904No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4240.1712806052991957No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3570.14421503795238882No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3550.14340711056890207No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3480.14057936472669838No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3330.13451990935054758No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3250.13128819981660048No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3090.1248247807487063No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3080.12442081705696292No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2980.12038118013952906No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2930.11836136168081213No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.11755343429732536No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2880.11634154322209521No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2810.1135137973798915No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2790.11270586999640472No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2780.11230190630466133No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2710.10947416046245763No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2630.10624245092851053No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2630.10624245092851053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG5150.037.410964
ATGGGTA3050.036.9771735
GTATCAA8550.036.3404581
GTACATG16300.034.080581
TGGGTAC3450.034.0520446
ACATGGG16250.033.834113
TACATGG16400.033.524652
GGGTACC3300.032.7518737
CATGGGT4750.032.6469464
GGTACCT3200.032.3264668
AACGCAG10200.031.3278796
CAACGCA10350.029.9657975
TCAACGC10550.028.9523054
ATGGGGA3450.028.6037185
ATCAACG10750.028.4136583
ACGCAGA11550.027.6661787
GTATATA1205.7449033E-627.4618261
CGCAGAG11650.027.4453358
GTATAGA700.00875231926.901381
GGATTAT700.00875231926.901381
Produced by FastQC (version 0.11.2)