FastQCFastQC Report
Sun 14 Apr 2019
SRR6576434_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576434_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345106
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7980.23123330223177807No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4720.13676957224736747No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4560.13213331556101604No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4140.11996314175934351No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4100.11880407758775564No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4080.11822454550196172No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3850.11155992651533152No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3790.10982133025794973No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3520.10199764709973168No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAC250.00523511656.3940855
GTATCAA7900.037.487951
ATGGGTA3450.036.778755
CATGGGT5000.036.656164
TACATGG21100.036.3147772
GTACATG21500.035.8577961
ACATGGG21650.035.1649063
GTAACAC702.1831914E-433.567913
TACTCCG600.00415911831.330055
GGGTACC3550.030.4475127
ATGCACC1701.2187229E-1030.4085774
CATGGGG10300.030.1133484
GGTACCT3550.029.1237078
TATAAGC650.006142727628.9284292
CAACGCA10100.028.8484635
AACGCAG10050.028.5243726
ATCAACG10250.027.96783
TCAACGC10350.027.697584
TAGACCC700.00883212526.8543284
TGGGTAC4300.026.229816
Produced by FastQC (version 0.11.2)