FastQCFastQC Report
Sun 14 Apr 2019
SRR6576435_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576435_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308894
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8280.2680531185455205No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5000.16186782520864762No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4450.1440623644356964No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4210.13629270882568129No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3710.12010592630481655No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3690.11945845500398194No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3390.1097463854914631No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3310.10715650028812473No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3210.10391914378395177No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3110.10068178727977882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7800.044.0162961
GTACATG17100.038.7800371
TACATGG17550.037.5176962
ACATGGG18150.036.5010763
ATGGGTA2800.035.2390755
AACGCAG9600.034.260216
GGGTACC2700.033.063827
CAACGCA10150.032.8666535
ATCAACG10350.032.6855163
GGTACCT2750.032.462668
TCAACGC10500.031.7711014
ATAAGCC600.004162627331.3236183
CATGGGG8700.029.7034324
ACGCAGA11250.029.2353787
TGGGTAC2900.029.1633726
CATGGGT4600.028.5998274
CGCAGAG11850.027.7551068
CATGGGA7750.027.2818624
TTATATA1057.371021E-526.8749242
TCTTAGA700.0087977526.8749222