FastQCFastQC Report
Sun 14 Apr 2019
SRR6576436_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576436_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257818
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6680.25909750288963534No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6320.24513416441055316No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4690.1819112707413757No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4500.1745417309885268No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4120.159802651482829No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3910.15165737070336438No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3820.14816653608359384No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3610.14002125530412926No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC3550.13769403222428223No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3440.13342745657789604No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3420.13265171555128036No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3230.12528217579843146No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2960.11480967193911984No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2850.11054309629273364No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2770.10744013218627095No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG2750.10666439115965526No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2710.1051129091064239No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG2700.10472503859311608No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2590.10045846294672986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8400.056.5428241
CCGAACT504.5325396E-756.4308551
GTACTAA352.9253153E-453.743671
CCGCTAA352.9337383E-453.7124024
CCGAACA405.653213E-447.0257151
ACTAAAC405.6694617E-446.9983523
CGAACTC503.0035071E-546.998352
CAGTAAG450.001009768941.8006321
CGCTAAA450.001012665941.776315
GGACCGT450.001012665941.776316
GTACATG19800.039.1880951
CCCGCTA608.806863E-539.1652953
GGGTACC2200.038.4531977
TACATGG20350.038.106772
CATGGGT3650.037.3411564
ACATGGG20850.037.192943
ATGGGTA2600.036.1525765
ATCAACG13100.035.8766023
TCAACGC13350.035.5568054
CAACGCA13450.035.2924425
Produced by FastQC (version 0.11.2)