FastQCFastQC Report
Sun 14 Apr 2019
SRR6576437_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576437_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341695
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8280.24232136847188282No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4290.12555056409956247No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4200.12291663618138983No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3770.11033231390567612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8750.038.6738621
ACATGGG17750.037.0700153
GTACATG18200.036.6699331
CTAGTAC651.4115022E-436.1534543
TACATGG18600.035.6286472
CATGGGA9050.032.1985444
ATGGGAC2400.031.3329935
TGGGTAC2850.031.3329936
GTATTAC804.7742395E-429.374681
ATGGGTA2900.029.1720965
TCAACGC11400.028.4470584
AACGCAG11250.028.4085796
CAACGCA11550.028.0776185
CATGGGT4400.027.7724254
ATCAACG11750.027.5996993
GGGTACC3150.026.856857
TATACTT1057.40713E-526.8568485
CATGGGG7300.025.1093164
TATCAAC14150.024.2470862
ACGCAGA13300.024.0298147
Produced by FastQC (version 0.11.2)