FastQCFastQC Report
Sun 14 Apr 2019
SRR6576437_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576437_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341695
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7490.21920133452347854No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4450.1302331026207583No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4250.12437992946926352No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3840.11238092450869927No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3440.10067457820570977No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3420.1000892608905603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8100.042.4563831
GGTATAG500.001681087737.687311
GTACATG20850.036.1508981
TACATGG20700.035.6464542
ATGGGTA3300.035.6051755
ACATGGG20750.035.107563
ATCAACG10400.032.9895633
TCAACGC10550.032.520524
AACGCAG10900.031.9074616
CAACGCA11000.031.6173935
CATGGGT5400.028.7215084
TATCAAC12550.028.086952
CATGGGG8950.027.3065814
ACGCAGA13000.027.1147087
CGCAGAG13000.027.1147088
GGGTACC3300.027.059937
CCCGATT350.00883463226.85647478-79
GGTACCT3350.026.6560528
GTCATAC909.376105E-426.1717431
CATGGGA10000.025.379374
Produced by FastQC (version 0.11.2)