FastQCFastQC Report
Sun 14 Apr 2019
SRR6576443_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576443_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422197
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8820.2089072162995No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4970.11771755839098809No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4870.11534899584790985No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4490.10634845818421258No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.10113762058944048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8750.041.3681871
GTACATG21700.040.510321
TACATGG22250.039.5089382
ACATGGG22700.037.4743083
CATGGGT5650.034.9366874
GTCTTAC702.1801349E-433.578071
TCAACGC11050.032.749854
AACGCAG10950.032.190526
ATCAACG11100.032.178923
CAACGCA11250.031.7498725
TATCCGT600.00415882231.3321116
CATGGGG11600.030.3867454
GTATAAG1252.3531175E-730.0859551
GGTACCT3550.027.8017318
ACGCAGA12750.027.6459777
ATGGGTA4450.027.4596025
ATGGGAG5100.026.7244455
TATCAAC13600.026.615562
CGCAGAG13550.026.0137428
TATAAGG1301.0840386E-525.31272
Produced by FastQC (version 0.11.2)