FastQCFastQC Report
Sun 14 Apr 2019
SRR6576446_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576446_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352776
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7040.19956006077510943No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4300.1218903780302515No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.1139533301585142No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3990.11310293217225661No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3860.10941787423180716No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3680.10431548631426174No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3600.10204775835090824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG20550.036.424021
GTATAGG1302.2555469E-1036.2125631
TACATGG20600.036.0405542
GTATCAA11550.035.460091
ACATGGG21150.034.2146343
AGCGTCC550.002706105434.2033278
CACCGTC550.002706105434.2033279
CATGGGG7500.032.579444
AACGCAG13150.031.0881636
ATCAACG13600.029.7143
TCAACGC13650.029.6051564
CAACGCA13850.029.5169225
GGTACCT2850.028.052738
CATGGGT4700.027.9937934
CCTATTA856.7423744E-427.6919561
CGCAGAG15000.027.5906858
ACGCAGA15000.027.5711297
CATGGGA11850.025.7748764
GGGTACC2950.025.4894897
ATGGGTA3550.025.1493535
Produced by FastQC (version 0.11.2)