FastQCFastQC Report
Sun 14 Apr 2019
SRR6576447_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576447_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207759
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3930.18916148036908148No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3520.16942707656467348No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2100.1010786536323336No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAC200.002156553370.5323261
CGAACTC353.628582E-667.1413042
GTATCAA5900.054.9913061
AATAACC405.666568E-446.9989172
CTTACAC608.8003304E-539.1657683
TAAGACT500.001699012237.5991334
TAGTCGG250.001700969537.59913340-41
TACATGG15150.037.537092
ACATGGG15050.037.474223
GTACATG15200.037.4316331
ATCAACG8700.036.7347833
TCAACGC8950.036.23384
CATGGGA8000.035.8366744
TATCAAC9200.035.2491872
CAACGCA9200.035.2491875
ATGGGAG3900.034.947915
CGCCGGT550.002712218334.181037
AACGCAG9450.033.8193246
GGTATCA2250.033.4375461
TGCCCGC753.2576095E-431.3477021
Produced by FastQC (version 0.11.2)