FastQCFastQC Report
Sun 14 Apr 2019
SRR6576447_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576447_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207759
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5790.27868828787200556No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3720.17905361500584813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2840.13669684586467976No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2570.123701018969094No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2400.11551846129409557No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2330.11214917283968444No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2170.10444794208674472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.1010786536323336No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGT250.005163931856.581451
GTATCAA7400.054.797351
ATCAACG9450.042.7654763
CAACGCA9550.041.8256035
TCAACGC9700.041.1788184
AACGCAG10100.039.5479746
GTACATG14500.039.021691
ATGGGAG2900.038.8901755
TATCAAC10550.038.3157422
TACATGG15150.037.540842
ACATGGG15050.036.532223
CATGGGA7950.035.4658854
GGTATCA3100.034.9831541
ATGGGAT1950.033.738065
ACGCAGA12100.033.3994837
CGCAGAG12400.032.591438
CGGGATA300.004161287631.328278-79
CATGGGT2800.030.2093334
GCAGAGT13900.029.074379
GTATATA650.00604703929.0161291
Produced by FastQC (version 0.11.2)