FastQCFastQC Report
Sun 14 Apr 2019
SRR6576448_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576448_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435815
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9130.20949255991647833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.10922065555338849No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4640.10646719364868121No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4560.10463155237887636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11700.046.6031271
ATCAACG14050.038.134813
CATGGGT5650.036.6013644
AACGCAG14650.036.5729756
TCAACGC14650.036.5729754
TACATGG24150.036.202512
CAACGCA14800.036.202315
GTACATG24550.035.995591
ACATGGG24000.035.4454423
TGGGTAC3200.032.3121456
ACGCAGA16450.032.285367
CGCAGAG16700.031.8020468
ATGGGTA3700.030.4861535
TATCAAC18050.030.4685342
GGGTACC3150.029.8409447
GGTACCT3200.029.3746788
GCAGAGT19550.027.4063479
GTATAGA1406.347327E-726.8599281
ATACCGT1258.009663E-626.3197126
CATGGGA13200.024.2118554
Produced by FastQC (version 0.11.2)