FastQCFastQC Report
Sun 14 Apr 2019
SRR6576449_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576449_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390264
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8490.21754504643010886No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5420.1388803476620954No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.12350613943381916No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4640.11889387696533628No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCG250.005236111656.3927737
GTATCAA10450.045.9226761
ATCAACG12300.037.4423563
AACGCAG12700.036.263076
CAACGCA12800.035.6126255
TCAACGC13050.035.2904974
GTACATG21250.034.3175351
TAGCGGT550.00270800334.1993459
TACATGG21200.033.6937982
ACATGGG21350.033.0168463
TATCAAC14650.032.07782
ACGCAGA15250.029.891257
CGCAGAG15550.029.3333648
CATGGGA11300.028.2795624
GTATAGT1005.2776224E-528.2289371
GATATAG856.767255E-427.6754321
GCGTTAT700.0087849426.8847031
GTACTAG700.0087849426.8847031
CTTATCG350.00883983526.85370322-23
CATGGGT5750.026.153174
Produced by FastQC (version 0.11.2)