FastQCFastQC Report
Sun 14 Apr 2019
SRR6576450_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576450_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522557
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9340.17873648233589828No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5640.10793080946193429No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5550.10620850931094597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGG33950.036.2693253
GTACATG34400.036.2185971
TACATGG34150.036.2083972
CATGGGT6050.033.4034274
CATGGGG11950.032.6428574
GTATCAA17050.029.7812941
ATGGGAG8100.027.8505765
CATGGGA20500.026.5939044
ATGGGGA6150.025.9825525
ATGGGTA4350.025.9298465
ATTATAC1901.33004505E-824.7357083
ATGGGGG5400.024.3692535
ATCAACG20950.024.0036743
AACGCAG21250.023.8859636
GGGTACC3750.023.8122437
TCAACGC21800.023.2833394
TGGGTAC4250.023.2224666
CAACGCA22250.022.8124395
GGTACCT4100.021.779498
CTAGTCC1303.1574367E-421.6913153
Produced by FastQC (version 0.11.2)