FastQCFastQC Report
Sun 14 Apr 2019
SRR6576451_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576451_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474194
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8290.17482296275364093No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5260.11092506442510872No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5190.10944887535481258No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4800.10122439339173418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17550.034.8271261
ACATGGG33600.033.1425253
TACATGG34250.032.9459532
GTACATG34350.032.7131651
GTATAGG600.004151347631.3444121
CATGGGT6850.029.4954094
AACGCAG21250.028.7449116
CATGGGA19650.027.9769154
CATGGGG11300.027.8594744
ATGGGTA4050.027.8440725
ATCAACG21800.027.5886233
TCAACGC22200.027.5148354
CAACGCA22350.027.3301735
ATGGGAG7250.027.2199825
ATGGGAT5000.026.3126475
GTATAAT2051.0732037E-925.228431
TATCAAC24050.025.2188912
ACGCAGA24400.025.0339897
CGCAGAG24550.024.8810338
ACTATAC1151.378153E-424.5148893
Produced by FastQC (version 0.11.2)