FastQCFastQC Report
Sun 14 Apr 2019
SRR6576452_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576452_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353257
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6090.1723957345502No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3680.10417344879223908No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3560.10077648850553562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAC751.4744E-743.8768421
GTATCAA8750.040.8323251
TACATGG20650.038.9291232
GTACATG20950.038.596061
ACATGGG20850.038.319383
AACGCAG10350.033.6021426
CATGGGT6300.033.569714
CATGGGG7450.033.434534
ATCAACG10450.033.2805943
TCAACGC10450.033.2805944
CAACGCA10550.032.9651375
GTATAGG1201.6434569E-731.3406031
GGGTACC3700.029.214727
TATCAAC12650.028.2437042
CATGGGA10500.028.1985574
ACGCAGA12550.028.086217
CGCAGAG12700.027.7544848
TGCGATA350.00883596326.85576884-85
ATGGGTA4300.026.2312155
GGTACCT4150.026.0468588
Produced by FastQC (version 0.11.2)