FastQCFastQC Report
Sun 14 Apr 2019
SRR6576452_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576452_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353257
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5760.16305409376176552No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3900.11040120931786207No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3740.1058719289355908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9500.037.1671641
GTACATG20200.034.726151
TACATGG20350.033.959352
ACATGGG21000.032.9082223
CGCGCTT300.004160579831.33229868-69
ATCAACG11950.029.5052833
CATGGGG7500.029.46074
TCAACGC12150.029.4065274
AACGCAG12400.028.8136546
CAACGCA12300.028.6657035
AACGCGA350.00883518626.85625540-41
ATGGGTA3550.026.4854935
ATGGGGG3200.026.444115
CATGGGA11150.025.2978064
TATCAAC13650.025.1418152
CGCAGAG14300.024.9781938
ACGCAGA14200.024.8231227
TATAGAG950.001300900724.7430275
CATGGGT5250.024.1774714
ATGGGAT3000.023.5058755
Produced by FastQC (version 0.11.2)