FastQCFastQC Report
Sun 14 Apr 2019
SRR6576453_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576453_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312505
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5930.1897569638885778No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.12223804419129293No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3530.11295819266891731No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3440.11007823874818004No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3250.10399833602662356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9000.040.275081
TACATGG16800.037.4876482
GTACATG17850.036.3939441
ACATGGG17150.035.9004483
AACGCAG10300.034.67926
ATCAACG10850.033.354443
CATGGGG7700.032.9606484
CAACGCA10850.032.9212655
TCAACGC11200.032.312114
GATACGC300.004159785331.3329660-61
ATGGGGA4700.029.9996435
ACGCAGA12300.029.4177067
TATCAAC12800.029.0074652
CGCAGAG12650.028.9938138
CTAATGC1005.3117412E-528.1996632
GCATAAT700.00875818126.8998681
GGGTACC2850.026.3814287
GCAGAGT14350.026.2143639
CCGTCCA909.476174E-426.1233439
TGGGTAC2900.025.9307276
Produced by FastQC (version 0.11.2)