FastQCFastQC Report
Sun 14 Apr 2019
SRR6576454_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576454_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506353
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10720.2117100125801565No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5470.10802740380722539No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5440.10743493175709436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10600.048.7771221
GTACATG28700.037.6682361
TACATGG28750.037.2757452
ATCAACG14800.035.5666353
ACATGGG30150.035.5413553
TCAACGC15050.035.288114
CAACGCA15450.034.98295
AACGCAG15650.034.5358356
CATGGGG12700.030.3456614
ACGCAGA18000.030.0269897
CGCAGAG18200.029.6970218
CATGGGA15400.028.9927444
TATCAAC19300.028.0072122
ATGGGGG5300.027.4898455
ATGGGAG7300.024.465115
GCAGAGT22250.024.2914969
AGAGTAC19600.023.97896210-11
ATGGGAC4350.022.689065
ATAACGC1252.4212664E-422.5594083
CTAAATC1303.1568325E-421.6917383
Produced by FastQC (version 0.11.2)