FastQCFastQC Report
Sun 14 Apr 2019
SRR6576454_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576454_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506353
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10060.19867562747727377No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6190.12224673301037023No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5180.10230017398929206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11300.040.8608671
GTACATG27550.034.2033231
TACATGG27500.033.49732
ACATGGG28650.032.9729583
ATCAACG14950.030.179813
TCAACGC15200.029.683434
CAACGCA15600.028.9223165
AACGCAG15600.028.9223166
CATGGGG11450.028.732874
ATGGGCG1503.6901838E-828.1992595
TCTAATG856.81183E-427.6463342
GTATAGA1807.2850526E-926.1750411
TATCAAC18000.026.1104262
TAGGTAG909.511759E-426.1104265
CATGGGT6750.025.7622874
GTCTAAT1101.000463E-425.6991331
CATGGGA15950.025.3410284
ACGCAGA18550.024.0694487
TTAGCCC1000.001757594623.4993844
ATGGGAG7850.023.3497055
Produced by FastQC (version 0.11.2)