FastQCFastQC Report
Sun 14 Apr 2019
SRR6576456_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576456_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379035
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5820.15354782539870987No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4100.10816943026369595No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3870.1021013890537813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG23700.034.8180161
GTATCAA12650.034.666241
TACATGG24200.033.6021922
ACATGGG23600.033.4606323
CGTCTAT600.00409164231.4356941
AACGCAG14000.030.8884896
CATGGGG9400.029.502654
ATCAACG14750.028.9992143
CAACGCA15050.028.7334795
GTCTAGA1154.0816485E-628.7021561
TCAACGC14800.028.5836494
TAGCCCT2203.6379788E-1227.7752254
ATGGGAG5200.027.1178235
TAAGGTA700.00882481826.8595564
TCCGGTC700.00883049926.8560128
ACGCAGA16150.026.7728677
CGCAGAG16350.026.7328198
ATGGGGA5350.026.3575085
TATAGAC1101.0159521E-425.6386682
ATAACGC1101.0159521E-425.6386683
Produced by FastQC (version 0.11.2)