FastQCFastQC Report
Sun 14 Apr 2019
SRR6576457_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576457_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352147
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6300.17890256057839482No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4290.12182412458433552No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4080.11586070589838904No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3700.10506975780000966No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3530.10024222838757677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG8450.041.1481974
CATGGGT5250.038.484424
GTACATG24000.036.6209451
TACATGG24050.036.5448072
ACATGGG23800.036.3259243
ATGGGGA4950.036.0706675
GTATTAT959.425712E-734.6316831
GTATCAA13100.032.6489521
GGTACCT2500.031.9510218
ATGGGTA3000.031.324535
ATAGGGG600.004162819631.324538
AACGCAG14450.029.2651316
TGGGTAC2600.028.914956
GGGTACC2650.028.3693857
TCCGACC856.8178267E-427.639298
TCAACGC15950.026.2183364
GATATAA909.504352E-426.111191
ATCAACG16200.026.1037753
CAACGCA16050.026.0549815
ACGCAGA16700.025.3222247
Produced by FastQC (version 0.11.2)