FastQCFastQC Report
Sun 14 Apr 2019
SRR6576458_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576458_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414800
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10070.24276759884281582No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5910.14247830279652843No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5720.13789778206364514No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5580.13452266152362582No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5150.12415621986499518No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4790.11547733847637415No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC4730.11403085824493733No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC4580.11041465766634523No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4390.1058341369334619No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4230.10197685631629702No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4220.10173577627772419No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4160.10028929604628738No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAT352.935505E-453.71359394
GTATCAA8800.044.371761
TTAGGAC450.001013269841.777243
CGAACTC608.80248E-539.175612
TCAACGC9750.039.0456544
ATCAACG10050.037.880113
TACATGG19300.037.754732
CAACGCA10150.037.506915
GTACATG19800.037.065571
ACATGGG19600.036.6883053
AACGCAG10650.035.304716
TAATACC801.1569688E-535.2495464
GTACTAG600.00413861331.3631761
AAACGCA600.004158205431.3329323
TTAAGTA1201.6481681E-731.3329324
GGTAATC953.740726E-529.6909892
ATGGGCG953.7468875E-529.6838285
ATAATAC953.7468875E-529.6838283
ACGCAGA12750.029.4898197
CCGAACT804.749126E-429.4029791