FastQCFastQC Report
Sun 14 Apr 2019
SRR6576458_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576458_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414800
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9260.2232401157184185No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6060.14609450337512053No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5360.1292189006750241No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5210.125602700096432No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5140.12391513982642237No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4600.11089681774349085No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4590.11065573770491803No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4470.10776277724204436No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4430.10679845708775314No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC4350.10486981677917068No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC4210.1014946962391514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9500.038.2352681
TACATGG20150.037.564622
GTACATG20250.037.5057531
ACATGGG20350.037.1819883
CATGGGG11200.033.1497424
TCAACGC11250.031.7491594
AACGCAG11450.031.605046
TATACGG600.004151839331.342742
GTATATC753.2731422E-431.3314063
CAACGCA11700.030.9297185
ATCAACG11650.030.6590583
GGGTATA953.6535585E-529.7937131
CTAGGGC650.006151546328.9212973
GGTACCT3550.027.8011058
TACGTGT1057.413445E-526.855494
CTTAGGT1956.020855E-1026.511193
GTTCCTA909.305175E-426.2074341
TTAGGTA1807.4724085E-926.1095034
ATGGGGG5400.026.1095035
ATGGGCG909.5108064E-426.1095035
Produced by FastQC (version 0.11.2)