FastQCFastQC Report
Sun 14 Apr 2019
SRR6576459_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576459_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487495
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9990.20492517871978175No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC6640.1362065251951302No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6560.13456548272289973No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5650.11589862460127796No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5630.11548836398322035No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5310.1089241940942984No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC4880.10010359080605954No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGAT250.00523428156.399884
GTATCAA13150.038.9620861
TACATGG27850.034.7683032
GTACATG27900.034.5375181
ACATGGG28750.033.3494953
AACGCAG15400.032.0453876
TAATAGT1807.2759576E-1231.3332674
CAACGCA15750.031.3332655
TCAACGC15650.031.2331584
ATCAACG15800.030.936643
CATGGGG11700.030.529854
CTTAGGT2203.6379788E-1227.7726673
TGGGTAC4100.027.5121356
GGTACCT4150.027.1806648
ACGCAGA18400.027.0760277
GGGTACC4200.026.8570847
ATATAAC1258.011662E-626.319943
GTATAAC909.504215E-426.1137331
CGCAGAG18900.026.1110538
TATCAAC19350.025.9922712
Produced by FastQC (version 0.11.2)