FastQCFastQC Report
Sun 14 Apr 2019
SRR6576460_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576460_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430869
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9700.22512643053921264No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6860.15921312510298954No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5550.1288094525250134No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC5310.12323931403744526No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5120.11882962106812048No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4730.10977814602582223No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4690.10884978961122754No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC4690.10884978961122754No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4330.10049458187987531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG23700.039.865091
GTCTTAG2400.039.1709251
TACATGG23950.038.8601722
ACATGGG24750.037.211293
TCTTAGG2500.035.723882
GTATCAA13450.035.2975161
GTACTAA801.1561082E-535.2538341
AGGTATA1601.8189894E-1235.2415586
CATGGGG10800.032.1959884
TAGGTAT2150.030.597325
AACGCAG15250.030.1960436
TTAGGTA2051.8189894E-1229.7977394
TCAACGC15700.029.031264
ATCAACG16050.028.398183
CAACGCA16150.028.2223425
CATGGGT7000.028.1932474
CTTAGGT2053.6379788E-1127.5056063
GTATAGT3250.027.4671548
CTATAAG1205.82714E-627.4196471
CGCAGAG17000.027.0844848