FastQCFastQC Report
Sun 14 Apr 2019
SRR6576460_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576460_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430869
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8950.20771974776556215No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6410.14876911543879925No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC6290.1459840461950152No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5220.12115051210460719No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.11256321526960632No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4660.10815352230028152No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC4650.10792143319663286No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4390.10188711650176735No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4360.10119084919082133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGT1850.038.1088143
GTATCAA13600.037.7618751
TACATGG25150.034.0165062
TAGGTAT1800.033.9450765
GTACATG25350.033.826681
GTCTAGG1001.3816389E-632.9810141
ACATGGG25800.032.244783
GTCTTAG2500.032.03871
TTAGGTA2000.030.5505664
CTAATAG953.7464168E-529.684763
AACGCAG16750.029.4598126
ATCAACG16950.029.3928763
CATGGGG11600.029.1729564
TGGGTAC3600.028.7194216
GGGTACC3600.028.7194217
GTATAGT2800.028.5528148
CAACGCA17300.028.5265395
TCAACGC17300.028.5265394
TATTATC4200.027.9799582
AGGTATA2400.027.4139926