FastQCFastQC Report
Sun 14 Apr 2019
SRR6576462_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576462_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253306
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5140.20291663047855166No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.17488729047081397No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3880.15317442145073548No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3310.1306719935571996No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3040.1200129487655247No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2960.1168547132716951No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2850.1125121394676794No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2640.10422177129637672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11800.039.8264921
GTACATG21200.035.468121
ATTCGCG401.1597393E-535.2394980-81
CAATTCG401.1597393E-535.2394978-79
AATTCGC401.1597393E-535.2394978-79
TACATGG21700.035.0840232
ACATGGG21700.034.427523
ATTATAC1256.2445906E-933.829913
CATGGGG8850.032.9167374
GAGTGCA1001.4086945E-632.890199
ATGGGGA4750.032.6428955
TCAACGC14350.032.4154174
ATCAACG14400.032.3028643
AACGCAG14300.032.2001886
CATGGGT4500.031.323994
CAACGCA15100.030.8053825
ATAATAC1252.3570465E-730.071033
GTCCTAT953.743435E-529.681221
ACGCAGA15600.029.5168367
TATCAAC16350.029.0307122