FastQCFastQC Report
Sun 14 Apr 2019
SRR6576463_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576463_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438819
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8180.18640943076758298No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6150.14014889966022437No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5210.11872776702923074No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4720.10756143193435108No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4390.10004124707453416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14350.042.721341
ATCAACG18000.033.680883
TCAACGC18650.032.759014
AACGCAG18250.032.704476
CAACGCA18800.032.2476545
ATGGGTA3850.031.737955
TACGGAC300.004159610331.33462570-71
ACGTCGC300.004159610331.33462566-67
TGATCGC600.004159684331.3310559
TACATGG27350.031.1019422
ACATGGG27450.030.817433
CATGGGT7500.030.7044334
GTACATG27800.030.5338171
GGGTACC3700.029.2140927
TATCAAC20950.029.1625582
GTGATCG650.006152264328.9209758
GGTACCT3600.028.7201338
GTCTAGA1154.0807245E-628.704741
ACGCAGA21100.028.2870457
CGCAGAG21800.027.5943248
Produced by FastQC (version 0.11.2)