FastQCFastQC Report
Sun 14 Apr 2019
SRR6576464_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576464_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401056
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7910.1972293146094311No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5500.13713795579669671No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5270.13140309582701667No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4810.11993337588765658No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4470.1114557568020426No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4170.10397550466767734No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4100.1022301125029921No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4060.1012327455517434No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA405.679992E-446.9870384
ACCGTGC753.277067E-431.324698
CGATAAT300.004165861331.3246966-67
CCCGCTA600.004163400731.324693
GTACATG28500.030.5231881
TACATGG28200.030.3476682
CATGGGT6400.030.101074
ATGGGTA3150.029.8330385
ACATGGG28550.029.624053
GACGTAA404.787336E-429.36689854-55
GTATCAA16450.028.8707771
GTATGAC700.00880896626.8698331
GGTACCT3150.026.8497338
CATGGGG10050.026.6493634
TACGCTA3250.026.0235889
GGGTACC3100.025.7670827
GTAATAG950.001299521424.7485311
AACGCAG19050.024.665116
TATAGGA1151.370622E-424.5333252
GTATGAA1351.4490526E-524.3818861
Produced by FastQC (version 0.11.2)