FastQCFastQC Report
Sun 14 Apr 2019
SRR6576464_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576464_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401056
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8210.2047095667437964No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5570.13888334796138194No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4640.11569456634484959No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4580.11419851591797654No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4450.11095707332641824No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4350.10846365594829649No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4080.10173142902736776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT200.002163383670.488217
GTATCAA16200.031.9680291
GTACATG29200.031.7630041
ACATGGG27700.031.7239383
TACATGG28800.031.3359132
GTTCTAA1551.4861143E-930.3743171
CATGGGT6500.028.1952864
CATGGGA15450.027.3740654
AACGCAG18400.027.3269566
GTATTAC700.00875617626.9029661
GGACTAA700.00875617626.9029661
CAGTACG1057.386747E-526.8694049
ATGGGTA3900.026.5083895
ATGGGAG6150.025.9793975
ATCAACG19600.025.6538753
ACCGTCG1659.2450136E-825.6480678
CATGGGG9150.025.1651944
GGGTACC4700.024.9958237
CAACGCA19950.024.9682585
TCAACGC20150.024.7204344
Produced by FastQC (version 0.11.2)