FastQCFastQC Report
Sun 14 Apr 2019
SRR6576469_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576469_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283072
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5890.20807427085688446No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.11940425050870451No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2910.10280070088175446No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2900.10244743386841511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6800.058.252151
TCAACGC9350.042.7256664
CAACGCA9300.042.4500165
AACGCAG9450.041.2788736
ATCAACG9500.041.0616153
TATCAAC10850.035.9589162
CGCAGAG11750.033.1987538
ACGCAGA11850.032.9185947
TACATGG13750.032.1353762
ACATGGG14700.031.6518733
CCTAGGT600.004156497331.3321553
CCTAACC600.004156497331.3321553
CTACGCT600.004156497331.3321554
TAAGACT902.587741E-531.3321554
GTACATG14300.031.3277471
GCAGAGT13000.030.0065639
CTAACCT804.772938E-429.3738964
ATACAGT804.772938E-429.3738966
CAGAACG650.00614757128.921994
ACCTAAG856.67232E-427.739121
Produced by FastQC (version 0.11.2)