FastQCFastQC Report
Sun 14 Apr 2019
SRR6576471_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576471_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241150
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7810.32386481443085213No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5260.21812150114036907No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5170.21438938420070497No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4750.19697283848227246No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4710.19531411984242175No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4530.18784988596309352No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4290.1778975741239892No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4220.17499481650425047No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4180.17333609786439974No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4110.170433340244661No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4100.17001866058469833No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3590.14886999792660172No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3560.14762595894671368No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3540.1467965996267883No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3480.14430852166701225No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3340.13850300642753474No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3070.1273066556085424No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG2860.11859838274932614No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2850.11818370308936346No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2800.11611030478955006No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2750.11403690648973668No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2690.11154882852996059No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA2680.11113414886999792No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG2680.11113414886999792No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2650.10989010989010989No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC2610.10823139125025918No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG2610.10823139125025918No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG2570.10657267261040845No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2540.10532863363052042No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT2530.10491395397055775No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG2520.10449927431059507No Hit
CTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTA2440.10118183703089363No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2420.10035247771096828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACA602.5480404E-854.8242654
CCTTATA555.275786E-542.7202032
GGTTAGT459.987898E-441.892471
ATAGGTC450.00101318541.770873
GGGTACC2350.039.993387
TAGGCAT500.001700743537.593785
ATGGGTA2500.037.5937775
GGTACCT2400.037.233068
GTATCAA12900.034.7074241
GTACATG20550.034.400751
CAAGACT550.002714974634.1761634
TGGGTAC2650.033.692546
ACATGGG20800.033.4367753
CATGGGT4100.033.2384034
CATGGGG6700.032.964694
TACATGG21300.032.8724942
TAATACC600.00415788931.3281524
AACGCAG14950.030.175616
GTACCTG2850.029.7039349
CAACGCA16100.028.020215
Produced by FastQC (version 0.11.2)