FastQCFastQC Report
Sun 14 Apr 2019
SRR6576472_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576472_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513096
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15430.30072345136192835No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA8290.1615682055599732No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8270.1611784149554859No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8150.1588396713285623No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7430.1448072095670206No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA7070.13779097868624976No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7010.13662160687278796No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT6370.12414830752919533No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6160.12005550618207898No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6110.1190810296708608No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5700.11109032227887179No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG5650.11011584576765361No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT5590.1089464739541918No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5520.10758220683848636No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT5280.10290471958463913No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC5270.10270982428239549No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC5270.10270982428239549No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA5160.10056597595771552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG33900.043.1374931
TACATGG35100.041.662712
ACATGGG34800.041.461183
GTATCAA13550.040.601381
ATGGGTA6950.037.1929325
CATGGGT10500.035.3606574
GGGTACC5750.034.3292277
GGTACCT5850.033.74248
ATCAACG16350.032.769493
TCAACGC16400.032.669584
CAACGCA16650.032.461325
CATGGGA17550.032.135624
AACGCAG16700.032.0827036
CATGGGG10850.031.6210044
CATGGGC5750.030.2424144
GTACCTG6850.029.502619
TGGGTAC6750.028.5471446
ACGCAGA18900.028.0995377
CGCAGAG19350.027.2031758
TTGGCGA350.008835006526.85750652-53
Produced by FastQC (version 0.11.2)